Summary of Progress

During Stage 1 of the FBREW project, all outcome indicators anticipated in the funding application were fully achieved, namely:

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✔ 1 / 1 ISI Paper submitted for publication and published:

1. Published article: Adsorption of Bisphenol A from Water Using Chitosan-Based Gels, Ludmila Aricov, Anca Ruxandra Leontieș*, Gels, 237, 11, 3, 180 (IF 5.3)

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✔ 5 / 2 International conference presentations:

1. Modulation of Trametes versicolor laccase activity with sodium sulfate and its potential application in the degradation of naphthol green, L. Aricov*, R. M. Toma, A. Raducan, A. Precupaş, A.R. Leontieș, 27th Materials, Methods & Technologies, Burgas, Bulgaria (poster).
2. Catalytic decomposition of vegetable oil using nanoreactors with entrapped lipase and additives, Anca-Ruxandra Leontieș*, Aurica Precupas, Raluca-Marieta Toma, Adina Raducan, Ludmila Aricov, MEDICTA 2025, Thessaloniki, Greece (poster).
3. Olive oil degradation using nanoreactors with entrapped lipase and additives, Leontieș Anca-Ruxandra*, Precupas Aurica, Toma Raluca-Marieta, Răducan Adina, Aricov Ludmila, 27th Materials, Methods & Technologies, Burgas, Bulgaria (poster).
4. Optical Microscopy Coupled with DSC: Insights into Composite and Advanced Materials, Raul Augustin Mitran, 8th Central and Eastern European Conference on Thermal Analysis and Calorimetry, Mostar, Bosnia and Herzegovina (oral presentation).
5. Nonessential activation of Laccase from Trametes versicolor with sodium sulfate, Ludmila Aricov*, Raluca-Marieta Toma, Adina Raducan, Aurica Precupas, Anca-Ruxandra Leontieș, MEDICTA 2025, Thessaloniki, Greece (poster).

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✔ 1 / 1 Patent submitted to OSIM

Mixed polymeric materials for enzymatic immobilization, Ludmila Aricov, Anca Ruxandra Leontieș, and Raul Augustin Mitran, submission code A2025/00564

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✔ 1 / 1 Participation in an Innovation Exhibition

Gold Medal at the UGAL INVENT Innovation and Research Exhibition (23–24 October 2025), Galați, awarded to ARCIF AG SRL together with the “Ilie Murgulescu” Institute of Physical Chemistry for the project “Design and construction of a fluidized bed reactor with co-immobilized enzymes for the removal of bisphenols and dyes from wastewater (FBREW)” – Andrei Terente, Puiu Ciobanu, Dragoș Ionuț Sandu, Ludmila Aricov, Anca Ruxandra Leonties.

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Additionally, the following materials were obtained:

• Functionalized rPET; thin Cs-PAA films deposited on functionalized rPET; well-characterized immobilization support (rPET-Cs-PAA).
• Biocatalyst rPET-Cs-PAA-Lc obtained; complete study of pH, temperature, reuse, and storage effects on the kinetic profiles of the immobilized enzyme using ABTS.
• Biocatalyst rPET-Cs-PAA-Tyr obtained; complete study of pH, temperature, reuse, and storage effects on the kinetic profiles of the immobilized enzyme using ABTS.
• Biocatalyst rPET-Cs-PAA-Tyr-Lc obtained; complete study of pH, temperature, reuse, and storage effects on the kinetic profiles of co-immobilized Lc and Tyr using ABTS and L-tyrosine.

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Furthermore, the following were developed and delivered:

• Reactor geometry version 1 established.
• Components purchased/manufactured and assembly of reactor version 1 completed.

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Additionally, the following materials and solutions were characterized:

• Formulated and characterized synthetic wastewater.
• Kinetic characterization of the product in synthetic wastewater; degradation products identified.
• Real wastewater from Galați and Ilfov analyzed; quantities of bisphenols and dyes quantified.
• Kinetic characterization of the product in real wastewater; degradation products identified.
• Storage and reusability profiles evaluated; operational stability assessed.