In this stage, we aimed to develop, characterize, and test the operational stability of innovative materials based on immobilized enzymes on a recycled polyethylene terephthalate (rPET) support.
Objective O1: Preparation and Characterization of Enzymatic Materials
The main objective (O1) was to prepare and characterize rPET supports with chemically immobilized enzymes (laccase and tyrosinase).
A1.O1: Involved the functionalization of recycled rPET and subsequent coating with polyacrylic acid (PAA) and chitosan (Cs), yielding the rPET-Cs-PAA support.
A2.O1 and A3.O1: Focused on the separate immobilization of laccase and tyrosinase, respectively, onto this support.
A4.O1: Focused on the co-immobilization of both enzymes onto the same support, resulting in the composite material rPET-Cs-PAA-Lc-Tir.
Objective O2: Operational Stability Evaluation
The goal of Objective O2 was to evaluate the operational stability of the co-immobilized product (rPET-Cs-PAA-Lc-Tir) in the treatment of both synthetic and real wastewater.
A1.O2: Evaluated the efficiency of the co-immobilized enzymes in the treatment of synthetic wastewater containing bisphenols and dyes.
A2.O2: Consisted of obtaining and analyzing the water profile at the outlet of treatment plants in Ilfov and Galați.
A3.O2: Completed this section by evaluating the performance of the co-immobilized enzymes in real wastewater sourced from the mentioned plants.
Note: Objective O2 was partially achieved in this stage, excluding activities O2.A2.T3 and O2.A3.T3/T4.
Objective O3: Reactor Design
Objective O3 aimed to design a laboratory-scale fluidized bed reactor (FBR) using the co-immobilized enzymes.
A1.O3: Involved developing the conceptual geometry design for a 2 L reactor.
A2.O3: Consisted of fabricating the first version of this reactor.
Note: Objective O3 was partially completed in this stage, excluding activity O3.A2.T3.
No comments:
Post a Comment