Stage Summary

 

Research Stage (as per Funding Application): Stage 1.

Stage Title: Obtaining and Characterizing Co-immobilized Tyrosinase and Laccase on Recycled Polyethylene Terephthalate Coated with Polyacrylic Acid and Chitosan; Operational Stability of the Co-immobilized rPET-Cs-PAA-Lc-Tyr Product in Synthetic and Real Wastewater (PARTIAL); Design of a Laboratory-Scale Fluidized Bed Reactor with Co-immobilized Enzymes for Wastewater Treatment Applications (PARTIAL).

Period of Activities for this Research Report: January 2025 – December 2025 (12 months).

Stage Activities:

O1. Obtaining and Characterizing Co-immobilized Tyrosinase and Laccase on Recycled Polyethylene Terephthalate Coated with Polyacrylic Acid and Chitosan

• A1. Production of recycled polyethylene terephthalate (rPET) coated with polyacrylic acid and chitosan.
• A2. Immobilization of Lc on rPET-Cs-PAA.
• A3. Immobilization of Tyr on rPET-Cs-PAA.
• A4. Co-immobilization of Lc and Tyr on rPET-Cs-PAA.

O2. Operational Stability of the Co-immobilized rPET-Cs-PAA-Lc-Tyr Product in Synthetic and Real Wastewater (Stage 1 excludes O2.A2.T3 and O2.A3.T3/T4)

• A1. Evaluation of the performance of co-immobilized enzymes in synthetic wastewater containing bisphenols and dyes.
• A2. Analysis of water at the outlet of wastewater treatment plants in Ilfov and Galați.
• A3. Evaluation of the performance of co-immobilized enzymes in real wastewater from the Ilfov and Galați treatment plants.

O3. Design of a Laboratory-Scale Fluidized Bed Reactor with Co-immobilized Enzymes for Wastewater Treatment Applications (Stage 1 excludes O3.A2.T3)

• A1. Conceptual geometry design of the 2 L FBR.
• A2. Fabrication of Reactor Version 1.

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Verifiable Outcomes of Activities

• Project website development.
• 1 ISI article submitted for publication.
• 1 patent application submitted for evaluation.
• Participation in 2 scientific conferences.
• Participation in 1 innovation exhibition.
• Functionalized rPET; Thin films of Cs-PAA deposited on functionalized rPET; Well-characterized immobilization support (rPET-Cs-PAA).
• rPET-Cs-PAA-Lc biocatalyst obtained; complete study of the influence of pH, temperature, reuse, and storage on the kinetic profiles of the immobilized enzyme using ABTS.
• rPET-Cs-PAA-Tyr biocatalyst obtained; complete study of the influence of pH, temperature, reuse, and storage on the kinetic profiles of the immobilized enzyme using ABTS.
• rPET-Cs-PAA-Tyr-Lc biocatalyst obtained; complete study of the influence of pH, temperature, reuse, and storage on the kinetic profiles of co-immobilized Lc and Tyr using ABTS and L-tyrosine.
• Reactor geometry (Version 1) established.
• Components acquired/fabricated and assembly of Reactor Version 1.
• Synthetic wastewater formulated and characterized.
• Product kinetically characterized in synthetic water; degradation products identified.
• Real wastewater from Galați and Ilfov analyzed; quantity of bisphenols and dyes quantified.
• Product kinetically characterized in real wastewater.